Purdue Center for Macromolecular Crystallography

Overview of Services

The Purdue Center for Macromolecular Crystallography (PCMC) provides instrumentation and expertise used by researchers to perform high-throughput crystallization screening, data collection, and structure determination of proteins, viruses, and nucleic acids. The PCMC consists of a crystallization core in HOCK 284 with state-of-the-art automated instrumentation for growing crystals, and a diffraction core in 289 with a newly installed X-ray diffractometer for rapid in-house data collection on crystals for structure determination. The PCMC also offers staff assistance and training and we can also collaborate on projects for non-crystallographers.

Getting Started

Contact the Lab manager Tim Schmidt (tschmidt@purdue.edu) or Nick Noinaj (nnoinaj@purdue.edu) to initiate projects and for training. For those with little or no background in X-ray crystallography, assistance in understanding the basic fundamentals of X-ray crystallography is also provided.

Leadership

Dr. Nick Noinaj - Director

765-496-0061

nnoinaj@purdue.edu

Tim Schmidt - X-Ray Lab Manager

765-496-3131

tschmidt@purdue.edu

Location

Hockmeyer Hall of Structural Biology

Rooms 284 (Crystallization Core), 289 (X-ray Diffraction Core), 307 (Mosquito-LCP), Chirascan (HOCK 163C)

240 South Martin Jischke Drive

West Lafayette, IN 47907

iLab

**For Purdue individuals: First-time users should click "Sign-up" in upper right. Returning students will click "Login"

**For Non-Purdue individuals: Before you register, you must be assigned a Business Partner Number and given Purdue credentials to register/login.

Please contact the center manager for further information.

Contacts

Name	Role	Phone	Email	Location
Tim Schmidt	Lab Manager	765-496-3131	tschmidt@purdue.edu	HOCK 320
Nick Noinaj	Director	765-496-0061	nnoinaj@purdue.edu	HOCK 333

Equipment Usage Rates

Name	Description	Price
Crystallization Tray Singles (HOCK 307) ::	96-well hanging drop crystallization plates including: MCSG-1, MCSG-2, MCSG-3, MCSG-4, ShotGun SG1, LMB, TOP-96, AmSO4, Index HT, PEGs, PEGs II, PEG/Ion HT, Morpheus HT, MidasPlus HT, Wizard 1+2 HT, Wizard 3+4 HT, MPD, SaltRx HT, ProPLex HT, PACT premier HT, MemGold HT, MemGold 2 HT, MemFacHT, Memstart/Sys $40/plate; users must provide their own crystallization cover sheets or purchase from the PCMC.	Inquire
Incubator Room Storage Bin (HOCK 284) ::	Crystallization tray incubator storage bins (cubbies) at 4, 12, and 20 deg C. Each storage bin is charged $2.34/mo.	Inquire
Liquid Nitrogen Fill Station (HOCK 289) ::	Use the log sheet to monitor how much liquid nitrogen you use. This will be billed monthly. Fee :: $1.06/L.	Inquire
Rock Imager 1000 Duo (Formulatrix) (HOCK 284) ::	This robot is used for automated imaging and scoring of 96-well crystallization plates (hanging drop and sitting drop) on a set schedule up to 30 days. This robot contains a plate hotel that will hold up to 960 plates for each temperature 4 deg C and 20 deg C. Plates must be manually barcoded and loaded/unloaded into either the 4 deg C or 20 deg C unit. A Rock Maker Web interface is used to access and view the images remotely. Fee for use :: $5.00/plate for 30 days.	Inquire
SETUP FEE :: Chirascan V100 Circular Dichroism Spectrometer (Applied Photophysics) ::	1-Time Setup Fee :: $29.28.	Inquire
SETUP FEE :: MicroMax 007 X-ray Diffractometer (Rigaku/Dectris) ::	1-Time Setup Fee :: $29.28.	Inquire
Training/Assisted Use Fee ::	Fee for training or other staff assistance; charge per hour. Charged at $58.55/hr.	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Active Services (7)
Crystallization Tray Singles (HOCK 307) ::	96-well hanging drop crystallization plates including: MCSG-1, MCSG-2, MCSG-3, MCSG-4, ShotGun SG1, LMB, TOP-96, AmSO4, Index HT, PEGs, PEGs II, PEG/Ion HT, Morpheus HT, MidasPlus HT, Wizard 1+2 HT, Wizard 3+4 HT, MPD, SaltRx HT, ProPLex HT, PACT premier HT, MemGold HT, MemGold 2 HT, MemFacHT, Memstart/Sys $40/plate; users must provide their own crystallization cover sheets or purchase from the PCMC.	Inquire
Incubator Room Storage Bin (HOCK 284) ::	Crystallization tray incubator storage bins (cubbies) at 4, 12, and 20 deg C. Each storage bin is charged $2.34/mo.	Inquire
Liquid Nitrogen Fill Station (HOCK 289) ::	Use the log sheet to monitor how much liquid nitrogen you use. This will be billed monthly. Fee :: $1.06/L.	Inquire
Rock Imager 1000 Duo (Formulatrix) (HOCK 284) ::	This robot is used for automated imaging and scoring of 96-well crystallization plates (hanging drop and sitting drop) on a set schedule up to 30 days. This robot contains a plate hotel that will hold up to 960 plates for each temperature 4 deg C and 20 deg C. Plates must be manually barcoded and loaded/unloaded into either the 4 deg C or 20 deg C unit. A Rock Maker Web interface is used to access and view the images remotely. Fee for use :: $5.00/plate for 30 days.	Inquire
SETUP FEE :: MicroMax 007 X-ray Diffractometer (Rigaku/Dectris) ::	1-Time Setup Fee :: $29.28.	Inquire
SETUP FEE :: Chirascan V100 Circular Dichroism Spectrometer (Applied Photophysics) ::	1-Time Setup Fee :: $29.28.	Inquire
Training/Assisted Use Fee ::	Fee for training or other staff assistance; charge per hour. Charged at $58.55/hr.	Inquire